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Active Recombinant Mouse Collagen, Type XIII, Alpha 1, His-tagged

Cat.No. : Col13a1-465M
Product Overview : Recombinant Mouse Col13a1protein was expressed in Murine myeloma cell line with a N-terminal 6-His tag.Glu107-Gln565.
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  • Gene Information
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Cat. No. : Col13a1-465M
Description : Col13a1 is an 85 kDa95 kDa protein in the type 2 transmembrane collagen family. Mature mouse Col13a1consists of a 40 amino acid (aa) cytoplasmic domain, a 19 aa transmembrane segment,and a 692 aa extracellular domain (ECD). The ECD contains three collagenous regionsseparated by shorter noncollagenous regions. Within comparable regions of theECD, mouse Col13a1 shares 85% and 88% aa sequence identity with human and ratCol13a1, respectively.
Source : Mammalian cells
Form : Lyophilized from a 0.2μm filtered solution in PBS.
N-terminalSequence : His
Molecular Weight : 46.0 kDa
Activity : Measuredby the ability of the immobilized protein to support the adhesion of A431 humanepidermoid carcinoma cells. When 1 x 105 cells per well are added tormCol13a1 coated plate (2 μg/mL, 100 μL/well), approximately 45%-75% will adhereafter 1 hour at 37°C. Optimal dilutions should be determined by each laboratoryfor each application.
Endotoxin Level : <1.0 EUper 1 μg of the protein by the LAL method.
Purity : >80%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstituteat 300 μg/mL in PBS
Storage : Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
Gene Name : Col13a1 collagen,type XIII, alpha 1 [ Mus musculus ]
Official Symbol : Col13a1
Synonyms : Col13a1; collagen,type XIII, alpha 1; collagen alpha-1(XIII) chain; type XIII collagen; procollagen,type XIII, alpha 1
Gene ID : 12817
mRNA Refseq : NM_007731
Protein Refseq : NP_031757
UniProt ID : Q9R1N9
Chromosome Location : 10 B4; 10 31.5 cM
Function : heparin binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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