Recombinant Mouse Fasl(Gln101-Leu279) fused with HA tag at N-terminal was expressed in CHO.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 1-8 ng/mL in the presence of 2.5 µg/mL of a cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.
Predicted Molecular Mass: 25.9 (monomer) kDa;
SDS-PAGE: 40-55 kDa, reducing conditions.
<0.01 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.