Active Recombinant Human Kallikrein B, Plasma (Fletcher factor) 1, His-tagged

• Cat.No.: KLKB1-483H
• Product Overview: Recombinanthuman KLKB1 protein with a C-terminal 6-His tag wasexpressed in Murine myeloma cell line, NS0-derived. Gly20 - Ala638.

Specification

• Cat. No.: KLKB1-483H
• Description: Human KLKB1, aserine protease, is synthesized in the liver and circulates in the plasma bybinding to high molecular weight (HMW) kininogen or as a free zymogen. Onceactivated by its physiological activator, factor XII, it displays endopeptidaseactivity towards peptide bonds after arginine (preferred) and lysine. Itcleaves HMW kininogen, its major physiological substrate, to release thepotent vasodilator peptide bradykinin.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in MES and NaCl.
• N-terminalSequence: Gly20
• MolecularWeight: 70 kDa
• Activity: Measured by itsability to cleave a flourogenic peptide substrate ProPheArg7amido4methylcoumarin(PFRAMC). The specific activity is > 1,000 pmoles/min/μg, as measuredunder the described conditions. See Activity Assay Protocol.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >90%, by SDSPAGE underreducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 500μg/mL in sterile 25 mM MES and 150 mM NaCl, pH 5.5.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: KLKB1 kallikrein B, plasma(Fletcher factor) 1 [ Homo sapiens ]
• Official Symbol: KLKB1
• Synonyms: KLKB1; kallikrein B,plasma (Fletcher factor) 1; PPK; KLK3; plasma kallikrein; kininogenin; Fletcherfactor; plasma prekallikrein; plasma kallikrein heavy chain; plasmakallikrein light chain
• Gene ID: 3818
• mRNA Refseq: NM_000892
• Protein Refseq: NP_000883
• MIM: 229000
• UniProt ID: P03952
• Chromosome Location: 4q35
• Pathway: Blood ClottingCascade; Complement and Coagulation Cascades; Formation of Fibrin Clot(Clotting Cascade); Hemostasis; Intrinsic Pathway
• Function: peptidase activity;protein binding; serine-type endopeptidase activity