Recombinant Human Histone Deacetylase 6, FLAG-tagged

• Cat.No.: HDAC6-398H
• Product Overview: Recombinanthuman HDAC6 was expressed in E. coli as the full length protein with anN-terminal FLAG tag. The molecular weight of the protein is 132 kDa.

Specification

• Cat. No.: HDAC6-398H
• Description: HDAC6is a histone deacetylase containig two HDAC catalytic domains. HDAC6 is anenzyme that in humans is encoded by the HDAC6 gene. Histones play a critical rolein transcriptional regulation, cell cycle progression, and developmentalevents. It contains an internal duplication of two catalytic domains whichappear to function independently of each other.
• Source: E. coli
• Species: Human
• Reconstitution: 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20%glycerol.
• Contents: 10g recombinant HDAC6 protein, 0.5 g/l.
• Dilution of Protein: RecombinantHDAC6 is supplied in solution and should be kept as a solution in ordertomaintain full activity.
• Application: RecombinantHDAC6 is suitable for use in histone deacetylase assays. Arecommendedstarting point for the HDAC assay is 20-100 ng of recombinant proteinperreaction.
• Storage: Recombinantproteins in solution are temperature sensitive and should be stored at-80°Cto prevent degradation. Avoid repeated freeze/thaw cycles and keep on icewhennot in storage.

Gene Information

• Gene Name: HDAC6 histonedeacetylase 6 [ Homo sapiens ]
• Official Symbol: HDAC6
• Synonyms: HDAC6;histone deacetylase 6; HD6; JM21; FLJ16239; EC 3.5.1.98; KIAA0901;OTTHUMP00000032398; OTTHUMP00000197663
• Gene ID: 10013
• mRNA Refseq: NM_006044
• Protein Refseq: NP_006035
• MIM: 300272
• UniProt ID: Q9UBN7
• Chromosome Location: Xp11.23
• Pathway: Cell cycle; NOTCH1 Intracellular DomainRegulates Transcription; Signal Transduction; Signaling by NOTCH; Signalingby NOTCH1; Signaling events mediated by HDAC Class I; Signaling eventsmediated by HDAC Class II; TNF-alpha/NF-kB Signaling Pathway
• Function: Hsp90 protein binding; NAD-dependenthistone deacetylase activity (H3-K14 specific); NAD-dependent histonedeacetylase activity (H3-K9 specific); NAD-dependent histone deacetylaseactivity (H4-K16 specific); actin binding; alpha-tubulin binding;beta-catenin binding; beta-tubulin binding; dynein complex binding; enzymebinding; histone deacetylase activity; histone deacetylase activity (H3-K16specific); histone deacetylase binding; hydrolase activity; metal ion binding