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Recombinant Human ERBB2

Cat.No. : ERBB2-2515H
Product Overview : RecombinantHuman ERBB2 protein, was expressed in Human HEK293 cells and purified bySDS-PAGE and Coomassie blue staining
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Cat. No. : ERBB2-2515H
Description : This gene encodes amember of the epidermal growth factor receptor (EGFR) family of receptortyrosine kinases. This membrane-bound protein has a neuregulin binding domainbut not an active kinase domain. It therefore can bind this ligand but notconvey the signal into the cell through protein phosphorylation. However, itdoes form heterodimers with other EGF receptor family members which do havekinase activity. Heterodimerization leads to the activation of pathways whichlead to cell proliferation or differentiation. Amplification of this gene and/oroverexpression of its protein have been reported in numerous cancers,including prostate, bladder, and breast tumors. Alternate transcriptionalsplice variants encoding different isoforms have been characterized. Oneisoform lacks the intermembrane region and is secreted outside the cell. Thisform acts to modulate the activity of the membrane-bound form. Additionalsplice variants have also been reported, but they have not been thoroughlycharacterized.
Host : Human HEK293 cells
Preparation : HEK293Tcells were transiently transfected with MegaTran Transfection Reagent and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrsbefore collection. The cells were lysed in modified RIPA buffer (25mMTris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitorcocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify thelysate. Recombinant proteins were purified through anti-DDK affinity columns.Protein concentration was measured by BCA kit.
Molecular Weight : 137.7 kDa
Purity : > 80%as determined by SDS-PAGE and Coomassie blue staining
Concentration : > 50ug/ml as determined by BCA
Buffer : 10%glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3
Storage : Store at-80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt ofproducts under proper storage and handling conditions
Gene Name : ERBB2v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian) [ Homo sapiens ]
Official Symbol : ERBB2
Synonyms : ERBB2; v-erb-b2 erythroblasticleukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogenehomolog (avian); NEU; NGL; HER2; TKR1; CD340; HER-2; HER-2/neu; Receptortyrosine-protein kinase erbB-2; p185erbB2; C-erbB-2; Tyrosine kinase-typecell surface receptor HER2; MLN 19; c-erb B2/neu prote;neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblasticleukemia viral oncogene; homolog 2 (neuro/glioblastoma derived oncogenehomolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2
Gene ID : 2064
mRNA Refseq : NM_004448
Protein Refseq : NP_004439
MIM : 164870
UniProt ID : P04626
Chromosome Location : 17q11.2-q12
Pathway : Adherens junction; Bladder cancer;Calcium signaling pathway; Endometrial cancer; ErbB signaling pathway; Focaladhesion; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer;Prostate cancer; Axon guidance
Function : ATP binding; ErbB-3 class receptorbinding; Hsp90 protein binding; epidermal growth factor receptor activity;glycoprotein binding; growth factor binding; identical protein binding;nucleotide binding; protein C-terminus binding; protein heterodimerizationactivity; protein phosphatase binding; protein phosphatase binding; receptorsignaling protein tyrosine kinase activity; transferase activity;transmembrane receptor activity; ubiquitin protein ligase bindin

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (8)

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Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate? 02/01/2023

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.

What is the primary role of ERBB2 in cell signaling? 08/12/2022

ERBB2 is a receptor tyrosine kinase involved in the regulation of cell growth and differentiation.

What therapeutic agents target ERBB2 in cancer treatment? 10/03/2020

Therapeutic agents targeting ERBB2 include trastuzumab and lapatinib.

In which types of cancer is ERBB2 commonly overexpressed? 05/12/2020

ERBB2 is commonly overexpressed in breast, ovarian, and stomach cancers.

How does ERBB2 overexpression affect patient prognosis in cancer? 05/04/2019

ERBB2 overexpression is often associated with aggressive disease and poor prognosis.

How can ERBB2 levels be assessed in a clinical setting? 08/27/2018

ERBB2 levels can be assessed using immunohistochemistry and fluorescence in situ hybridization (FISH) in tissue samples.

How is ERBB2 involved in cell growth and proliferation? 08/07/2018

It forms heterodimers with other ERBB receptors, activating pathways that promote cell growth and survival.

Are there known genetic mutations that lead to ERBB2 overexpression? 02/22/2018

Amplification of the ERBB2 gene leads to its overexpression in cancer cells.

Customer Reviews (3)

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Reviews
05/09/2022

    Worked as expected, satisfied.

    03/16/2022

      Reliable in protein assays.

      09/27/2020

        Quick and reliable shipping.

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