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Active Recombinant Human MMP14, His-tagged

Cat.No. : MMP14-161H
Product Overview : Recombinant human MMP14 containing 6*his tag at C­terminalwas expressed in mouse myeloma cell, 60 kD (518aa).
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Cat. No. : MMP14-161H
Description : Proteinsof the matrix metalloproteinase (MMP) family are involved in the breakdown ofextracellular matrix in normal physiological processes, such as embryonicdevelopment, reproduction, and tissue remodeling, as well as in diseaseprocesses, such as arthritis and metastasis. Most MMP's are secreted asinactive proproteins which are activated when cleaved by extracellularproteinases. However, the protein encoded by this gene is a member of themembrane-type MMP (MT-MMP) subfamily; each member of this subfamily containsa potential transmembrane domain suggesting that these proteins are expressedat the cell surface rather than secreted. This protein activates MMP2protein, and this activity may be involved in tumor invasion.
Source : Mammalian cells
Species : Human
N­terminalSequenceAnalysis : Ala21
Activity : Measured by its ability to cleave a fluorogenic peptide substrate Mca­KPLGL­Dpa­AR­NH2.The specific activity is >500 pmol/min/μg.
EndotoxinLevel : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >80%, as determined by SDS­PAGE.
Formulation : Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl, CaCl2, Glycer-ol.
Stability&Storage : Use amanual defrost freezer and avoid repeated freeze­thaw cycles. 6 months fromdate of receipt, ­70 °C as supplied. 3 months, ­70 °C under sterileconditions after opening.
OfficialSymbol : MMP14
Gene Name : MMP14 matrix metallopeptidase 14(membrane-inserted) [ Homo sapiens ]
Synonyms : MMP14; matrixmetallopeptidase 14 (membrane-inserted); 1; MMP-X1; MT-MMP; MT1MMP; MTMMP1;MT1-MMP; MT-MMP 1; matrix metalloproteinase-14; membrane type 1metalloprotease; membrane-type matrix metalloproteinase 1; membrane-type-1matrix metalloproteinase; MMP-14; EC 3.4.24.80; OTTHUMP00000027917; EC 3.4.24
Gene ID : 4323
mRNA Refseq : NM_004995
Protein Refseq : NP_004986
MIM : 600754
UniProt ID : P50281
Chromosome Location : 14q11-q12
Pathway : Activation of Matrix Metalloproteinases; Extracellularmatrix organization; GnRH signaling pathway
Function : calcium ion binding; integrin binding; metalloendopeptidaseactivity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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What is the cellular source of MMP14 and how is its expression regulated? 06/05/2022

MMP14, also known as matrix metalloproteinase 14, is primarily expressed by cells of mesenchymal origin, such as fibroblasts and endothelial cells. Its expression is tightly regulated at multiple levels, including transcriptional, post-transcriptional, and post-translational mechanisms. Transcriptional regulation involves the binding of various transcription factors, such as AP-1 and SP-1, to the MMP14 promoter region. Additionally, microRNAs and RNA-binding proteins can modulate its mRNA stability and translation.

What is the role of MMP14 in cancer progression and metastasis? 03/29/2021

MMP14 plays a crucial role in cancer progression and metastasis. It facilitates tumor invasion by degrading components of the extracellular matrix, promoting cancer cell migration, and aiding in angiogenesis. MMP14 also influences tumor cell behavior by interacting with other proteins involved in cell signaling pathways and epithelial-mesenchymal transition. Its overexpression is associated with poor prognosis in various types of cancer.

How is overexpression of MMP14 associated with tumor progression? 05/24/2020

Overexpression of MMP14 has been strongly associated with tumor progression and poor prognosis in various types of cancer. Increased levels of MMP14 lead to enhanced degradation of the ECM, promoting tumor cell invasion and metastasis. Additionally, MMP14 can activate other matrix metalloproteinases (MMPs), further amplifying the proteolytic activity and facilitating tumor growth and angiogenesis. Moreover, MMP14 has been implicated in tumor-associated angiogenesis and immunosuppression, contributing to tumor progression and metastasis.

Can MMP14 be targeted for therapeutic interventions? 12/10/2019

Targeting MMP14 holds potential for therapeutic interventions in cancer and other diseases. Strategies such as selective inhibition of MMP14 activity, antibody-based therapies, and gene silencing techniques are being explored. However, the development of MMP14-targeted therapies faces challenges, including the need for specificity, avoiding off-target effects, and optimizing delivery methods to reach the tumor microenvironment.

Are there any known endogenous inhibitors of MMP14? 03/25/2017

Yes, there are endogenous inhibitors of MMP14, known as tissue inhibitors of metalloproteinases (TIMPs). TIMPs bind to the active site of MMP14 and prevent its activity. TIMP-2 is a specific inhibitor of MMP14 and forms a complex with MMP14, regulating its enzymatic activity. The balance between MMP14 and TIMPs is crucial for maintaining tissue homeostasis and preventing excessive extracellular matrix degradation. Dysregulation of this balance can contribute to pathological conditions, including cancer progression.

What are the physiological functions of MMP14 in tissue remodeling? 08/29/2016

MMP14 plays a crucial role in tissue remodeling processes, such as embryogenesis, wound healing, and tissue repair. It is essential for the degradation and turnover of extracellular matrix components during tissue restructuring. MMP14 also influences cell behavior and migration during tissue development and regeneration. Additionally, it participates in the modulation of immune responses and angiogenesis, which are crucial for proper tissue remodeling.

How does MMP14 interact with its substrates? 07/09/2016

MMP14 possesses a catalytic domain responsible for substrate cleavage. It predominantly cleaves components of the extracellular matrix, such as collagen type I and type II. The interaction between MMP14 and its substrates occurs through specific binding sites within the substrate proteins. This interaction is facilitated by the hemopexin-like domain of MMP14, which acts as a docking site for substrate recognition and binding.

Customer Reviews (3)

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Reviews
06/06/2018

    The versatility of this reagent is evident in its capacity to cater to a plethora of sample types, offering a broad array of potential applications.

    07/17/2017

      Backed by its user-friendly nature, this protein reagent saves me considerable time and effort, making it highly suitable for fast-paced laboratory work.

      04/26/2017

        Brimming with enchantment, this experimental reagent illuminates your scientific odyssey.

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