Recombinant Human Amyloid Beta(A4) Precursor Protein, GST-tagged and His-tagged

• Cat.No.: APP-6866H
• Product Overview: A DNA sequence encoding the amino acids ( Asp 672 - Val 713 ) of human APP, corresponding to the Beta-amyloid protein 42, was fused with the N-terminal polyhistidine-tagged GST-tagged tag at the N-terminus. It consists of 278 amino acids and has a calculated molecular mass of 32.3 kDa.

Specification

• Description: APP is a single-pass type I membrane protein which belongs to the APP family. APP functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. It couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP.
• Source: E. coli
• Species: Human
• Tag: GST and His
• Predicted N Terminal: Met
• Form: Lyophilized from sterile PBS, 10 % glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Molecular Mass: The recombinant human APP / GST-tagged chimera consists of 278 amino acids and has a calculated molecular mass of 32.3 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions
• Protein length: 278aa
• Purity: >80 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 °C.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: APP amyloid beta (A4) precursor protein [ Homo sapiens ]
• Official Symbol: APP
• Synonyms: APP; amyloid beta (A4) precursor protein; AD1,Alzheimer disease; amyloid beta A4 protein; peptidase nexin II; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; alzheimer disease amyloid protein; cerebral vascular amyloid peptide; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• Gene ID: 351
• mRNA Refseq: NM_000484
• Protein Refseq: NP_000475
• MIM: 104760
• UniProt ID: P05067
• Chromosome Location: 21q21.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amyloids, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem
• Function: DNA binding; PTB domain binding; acetylcholine receptor binding; heparin binding; identical protein binding; peptidase activator activity; peptidase inhibitor activity; protein binding; receptor binding; serine-type endopeptidase inhibitor activity; transition metal ion binding