Mouse Monoclonal Antibody to Human Acetoacetyl-CoA Synthetase
Acetoacetyl-CoA synthetase, also known as Acyl-CoA synthetase family member 1, Protein sur-5 homolog, AACS, and ACSF1, is a member of the ATP-dependent AMP-binding enzyme family. AACS is highly expressed in kidney, heart and brain, but low in liver. AACS is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies. AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. AACS was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS is a novel cytosolic ketone body (acetoacetate)-specific ligase, the physiological role of which remains to be elucidated
Human AACS / ACSF1. No cross-reactivity with Baculovirus lysate in ELISA
Recombinant human AACS protein
Western blot; ELISA
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human AACS in WB. Using a DAB detection system, the detection limit for human AACS is approximately 4 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human AACS. The detection limit for human AACS is 0.039 ng/well.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, insect cell-derived, recombinant human AACS.The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 centigrade for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 centigrade to -70 centigrade. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.