Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Mouse Anti-Human Acetoacetyl-CoA Synthetase Monoclonal Antibody

Mouse Anti-Human Acetoacetyl-CoA Synthetase Monoclonal Antibody

   Share
"AACS" Related Products.
By Species
+Expand -Collapse
By Tag
His(6)
Cat.No.:
CAB11476MH
Product Overview:
Mouse Monoclonal Antibody to Human Acetoacetyl-CoA Synthetase
Antigen Description:
Acetoacetyl-CoA synthetase, also known as Acyl-CoA synthetase family member 1, Protein sur-5 homolog, AACS, and ACSF1, is a member of the ATP-dependent AMP-binding enzyme family. AACS is highly expressed in kidney, heart and brain, but low in liver. AACS is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies. AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. AACS was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS is a novel cytosolic ketone body (acetoacetate)-specific ligase, the physiological role of which remains to be elucidated
Specificity:
Human AACS / ACSF1. No cross-reactivity with Baculovirus lysate in ELISA
Immunogen:
Recombinant human AACS protein
Host:
Mouse
Isotype:
Mouse IgG2b
Species:
Human
Clone:
8I7G4F2
Applications:
Western blot; ELISA
Dilution:
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human AACS in WB. Using a DAB detection system, the detection limit for human AACS is approximately 4 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human AACS. The detection limit for human AACS is 0.039 ng/well.
Preparation:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, insect cell-derived, recombinant human AACS.The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 centigrade for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 centigrade to -70 centigrade. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.