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Mouse Anti-Human Acetoacetyl-CoA Synthetase Monoclonal Antibody

Cat.No.: CAB11476MH
Product Overview: Mouse Monoclonal Antibody to Human Acetoacetyl-CoA Synthetase
Antigen Description: Acetoacetyl-CoA synthetase, also known as Acyl-CoA synthetase family member 1, Protein sur-5 homolog, AACS, and ACSF1, is a member of the ATP-dependent AMP-binding enzyme family. AACS is highly expressed in kidney, heart and brain, but low in liver. AACS is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies. AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. AACS was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS is a novel cytosolic ketone body (acetoacetate)-specific ligase, the physiological role of which remains to be elucidated
Specificity: Human AACS / ACSF1. No cross-reactivity with Baculovirus lysate in ELISA
Immunogen: Recombinant human AACS protein
Host: Mouse
Isotype: Mouse IgG2b
Species: Human
Clone: 8I7G4F2
Applications: Western blot; ELISA
Dilution: Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human AACS in WB. Using a DAB detection system, the detection limit for human AACS is approximately 4 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human AACS. The detection limit for human AACS is 0.039 ng/well.
Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, insect cell-derived, recombinant human AACS.The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
Format: 0.2 μm filtered solution in PBS with 5% trehalose
Storage: This antibody can be stored at 2-8 centigrade for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 centigrade to -70 centigrade. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name: AACS acetoacetyl-CoA synthetase [ Homo sapiens ]
Official Symbol: AACS
Synonyms: AACS; acetoacetyl-CoA synthetase; ACSF1; acyl CoA synthetase family member 1; FLJ12389; SUR 5; protein sur-5 homolog; acetoacetate-CoA ligase; acyl-CoA synthetase family member 1; homolog of C. elegans supressor of ras 5 (sur-5); SUR-5;
Gene ID: 65985
mRNA Refseq: NM_023928
Protein Refseq: NP_076417
MIM: 614364
UniProt ID: Q86V21
Chromosome Location: 12q24.31
Pathway: Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem;
Function: ATP binding; acetoacetate-CoA ligase activity; butyrate-CoA ligase activity; ligase activity; nucleotide binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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