Rabbit monoclonal antibody to human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1.
Beta-1,4-galactosyltransferase 1, also known as B4GALT1, GGTB2 and beta4GalT1, is a single-pass type II membrane protein. B4GALT1 is a member of the glycosyltransferase 7 family. It is ubiquitously expressed but at very low levels in fetal and adult brain. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 is an enzyme that functions in the synthesis of glycoconjugates It has two flexible loops, one short and one long. Upon binding a metal ion and UDP-galactose, the loops change from an open to a closed conformation, repositioning residues to lock the ligands in place. B4GALT1 is one of the best-studied glycosyltransferases. It plays a key role in the synthesis of selectin ligands. B4GALT1 may play an important role in the inflammation reaction. Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D). CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation.
Human B4GALT1 / GGTB2. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Recombinant human B4GALT1 protein
Western Blot; ELISA
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human B4GALT1 in WB. Using a DAB detection system, the detection limit for human B4GALT1 is approximately 1 ng/lane under non-reducing conditions and 0.125 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human B4GALT1. The detection limit for human B4GALT1 is approximately 0.039 ng/well.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human B4GALT1 / GGTB2.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.