Rabbit Anti-Human BMP and Activin Membrane-Bound Inhibitor-Like Monoclonal Antibody

Cat.No.

CAB11480RH

Product Overview

Rabbit monoclonal antibody to human BMP and activin membrane-bound inhibitor-like.

Antigen Description

BMP and activin membrane-bound inhibitor homolog, also known as BAMBI and NMA, is a single-pass type I membrane protein which belongs to the BAMBI family. Members of the transforming growth factor-beta (TGF-beta) superfamily, including TGF-beta, bone morphogenetic proteins (BMPs), activins and nodals, are vital for regulating growth and differentiation. These growth factors transduce their signals through pairs of transmembrane type I and type II receptor kinases. BAMBI is a transmembrane glycoprotein related to the transforming growth factor-beta (TGF-beta)-family type I receptors and functions as a negative regulator of TGF-beta signaling during development. BAMBI is co-expressed with the ventralizing morphogen BMP4 during Xenopus embryogenesis and that it requires BMP signalling for its expression. BAMBI is induced by BMP signaling through the evolutionary conserved BMP-responsive elements in its promoter. Wnt/beta-catenin signaling activates transcription of BAMBI and that BAMBI expression is aberrantly elevated in most colorectal carcinomas. BAMBI stably associates with TGF-beta-family receptors and inhibits BMP and activin as well as TGF-beta signalling. BAMBI negatively regulates TGF-beta-family signalling by a regulatory mechanism involving the interaction of signalling receptors with a pseudoreceptor.

Specificity

Human BAMBI / NMA. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.

Immunogen

Recombinant Human BAMBI / NMA Protein

Host

Rabbit

Isotype

Rabbit IgG

Species

Human

Clone

212

Applications

Western Blot; ELISA

Dilution

Western blot : This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human BAMBI in WB. Using a DAB detection system, the detection limit for Human BAMBI is approximately 10 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Direct ELISA : This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human BAMBI. The detection limit for Human BAMBI is approximately 0.00245 ng/well.

Preparation

This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human BAMBI / NMA.

Format

0.2 μm filtered solution in PBS with 5% trehalose

Storage

This antibody can be stored at 2-8 ^oC for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 ^oC to -70 ^oC. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Antigen Gene Information