Mouse monoclonal antibody to human basigin (Ok blood group).
CD147, also known as Basigin, BSG, and EMMPRIN, is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. CD147 is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation, and tumor progression. Enriched on the plasma memebrane of many human cancer (breast cancer, lymphoma, oral squamous cell carcinoma, glioma, melanoma, lung, bladder and kidney carcinomas) cells, CD147 induces the production and release of matrix metalloproteinases in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction.
Human CD147 / Basigin / BSG. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Recombinant human CD147 protein
Protein A affinity chromatography
Western Blot; ELISA; Immunohistochemistry
IHC-P: 5-20 μg/ml.Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CD147 in WB. Using a DAB detection system, the detection limit for CD147 is approximately 20 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditionsDirect ELISA: This antibody can be used at 0.5 - 1μg/mL with the appropriate secondary reagents to detect human CD147. The detection limit for human CD147 is 0.0195 ng/well
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CD147. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Immunochemical staining of human CD147 in human esophagus with mouse monoclonal antibody . The image showing membrane staining of squamous epithelium cell.