| Antigen Description : |
C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a exerts actions by binding to G-protein-coupled receptor (C5aR/CD88) on the plasma membrane of target cells. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimers disease, and sepsis. |
| Specificity : |
Human Complement C5a. No cross-reactivity in ELISA with Human Carbonic Anhydrase II / CA2, Human alpha-2-macroglobulin / A2M, E.coli cell lysate. |
| Immunogen : |
Recombinant Human Complement C5a Protein |
| Host : |
Mouse |
| Isotype : |
Mouse IgG2b |
| Species : |
Human |
| Clone : |
6F 2E2 |
| Purification : |
Protein A affinity chromatography |
| Applications : |
ELISA |
| Dilution : |
Direct ELISA: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect C5a-his. The detection limit for C5a-his is approximately 0.078 ng/well. |
| Preparation : |
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human Complement C5a. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Format : |
0.2 μm filtered solution in PBS with 5% trehalose |
| Storage : |
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
