Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Mouse Anti-Human Complement Component 5 Monoclonal Antibody

Mouse Anti-Human Complement Component 5 Monoclonal Antibody

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Human(15)
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Biotin(1)
His(3)
N/A(3)
Cat.No.:
CAB11483MH
Product Overview:
Mouse monoclonal antibody to human complement component 5.
Antigen Description:
C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a exerts actions by binding to G-protein-coupled receptor (C5aR/CD88) on the plasma membrane of target cells. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimers disease, and sepsis.
Specificity:
Human Complement C5a. No cross-reactivity in ELISA with Human Carbonic Anhydrase II / CA2, Human alpha-2-macroglobulin / A2M, E.coli cell lysate.
Immunogen:
Recombinant Human Complement C5a Protein
Host:
Mouse
Isotype:
Mouse IgG2b
Species:
Human
Clone:
6F 2E2
Purification:
Protein A affinity chromatography
Applications:
ELISA
Dilution:
Direct ELISA: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect C5a-his. The detection limit for C5a-his is approximately 0.078 ng/well.
Preparation:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human Complement C5a. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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