Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Rabbit Anti-Human Carbonic Anhydrase XIV Monoclonal Antibody

Rabbit Anti-Human Carbonic Anhydrase XIV Monoclonal Antibody

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GST(1)
His(6)
Cat.No.:
CAB11486RH
Product Overview:
Rabbit monoclonal antibody to human carbonic anhydrase XIV.
Antigen Description:
Carbonic anhydrases (CAs) are zinc-binding metalloenzymes that catalyze the reversible hydration of carbon dioxide to form HCO3- and protons. Fifteen CA isoforms have been reported sofar. CA I, CA II, CA III, CA VII, and CA XIII are cytosolic enzymes, whereas CA IV, CA IX, CA XII, CA XIV, and CA XV are membrane-associated isoforms. CA XIV is a polypeptide consisting of an extracellular N-terminal catalytic domain, a membrane-spanning segment and a short intracellular C-terminal segment with several potential phosphorylation sites. The CA XIV protein is expressed in the brain on plasma membranes of neuronal bodies and axons, in the kidney preferentially on the apical membranes of proximal tubules, and in the liver on the plasma membranes of hepatocytes. CA14, together with CA4, play important roles in the regulation of intracellular pH in hippocampal neurons by facilitating AE3-mediated Cl--HCO3- exchange.
Specificity:
Human Carbonic Anhydrase XIV / CA14. No cross-reactivity in ELISA with Human CARPX, Human CA12, Mouse CA14.
Immunogen:
Recombinant Human Carbonic Anhydrase XIV / CA14 Protein
Host:
Rabbit
Isotype:
Rabbit IgG
Species:
Human
Clone:
516
Applications:
Western Blot; ELISA; Immunohistochemistry
Dilution:
Immunochemistry : IHC-P: 2 - 8 μg/mL.Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human CA14 in WB. Using a DAB detection system, the detection limit for Human CA14 is approximately 0.25 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect Human CA14. The detection limit for Human CA14 is approximately 0.00245 ng/well.
Preparation:
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human Carbonic Anhydrase XIV / CA14.
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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