Rabbit monoclonal antibody to human carbonic anhydrase XIV.
Carbonic anhydrases (CAs) are zinc-binding metalloenzymes that catalyze the reversible hydration of carbon dioxide to form HCO3- and protons. Fifteen CA isoforms have been reported sofar. CA I, CA II, CA III, CA VII, and CA XIII are cytosolic enzymes, whereas CA IV, CA IX, CA XII, CA XIV, and CA XV are membrane-associated isoforms. CA XIV is a polypeptide consisting of an extracellular N-terminal catalytic domain, a membrane-spanning segment and a short intracellular C-terminal segment with several potential phosphorylation sites. The CA XIV protein is expressed in the brain on plasma membranes of neuronal bodies and axons, in the kidney preferentially on the apical membranes of proximal tubules, and in the liver on the plasma membranes of hepatocytes. CA14, together with CA4, play important roles in the regulation of intracellular pH in hippocampal neurons by facilitating AE3-mediated Cl--HCO3- exchange.
Human Carbonic Anhydrase XIV / CA14. No cross-reactivity in ELISA with Human CARPX, Human CA12, Mouse CA14.
Recombinant Human Carbonic Anhydrase XIV / CA14 Protein
Western Blot; ELISA; Immunohistochemistry
Immunochemistry : IHC-P: 2 - 8 μg/mL.Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human CA14 in WB. Using a DAB detection system, the detection limit for Human CA14 is approximately 0.25 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect Human CA14. The detection limit for Human CA14 is approximately 0.00245 ng/well.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human Carbonic Anhydrase XIV / CA14.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Immunochemical staining of human CA14 in human liver with rabbit monoclonal antibody . Positive staining was localized to membrane of hepatocyte.