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Home > Products > Antibody > Monoclonal Antibodies > Mouse Anti-Human CD244 Molecule, Natural Killer Cell Receptor 2B4 Monoclonal Antibody

Mouse Anti-Human CD244 Molecule, Natural Killer Cell Receptor 2B4 Monoclonal Antibody

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Product Overview:
Mouse monoclonal antibody to human CD244 molecule, natural killer cell receptor 2B4.
Antigen Description:
The CD244 antigen, also known as 2B4 / CD244, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. The 2B4 / CD244 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 / CD244 can effectively activate and enhance NK cell –mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a member of the CD2 subset of the Ig superfamily, CD244 is a high affinity ligand for CD48 and the interaction is essential for generation of NK effectors.
Human 2B4 / SLAMF4 / CD244. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. Human SLAMF1, Human cell lysate (293 cell line).
Recombinant Human 2B4 / SLAMF4 / CD244 Protein
Mouse IgG1
Protein A affinity chromatography
Western Blot
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human 2B4 in WB. Using a DAB detection system, the detection limit for Human 2B4 is approximately 20 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human 2B4 / SLAMF4 / CD244. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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