Rabbit monoclonal antibody to human CD27 molecule, FITC-conjugated.
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, and during which, adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling processes. In addition, a proapoptotic protein named SIVA, is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.
Human CD27 / TNFRSF7, Has cross-reactivity in ELISA with Mouse CD27. No cross-reactivity in ELISA with Human TNFRSF8/CD30, Human 4-1BB, Human CD40, Human DR6, Human FAS, Human HVEM/TNFRSF14, Human TNFRI, Human TNFRII, Human BAFF, Human RELT, Mouse HVEM, Mouse FAS, Rat FAS.
Recombinant human CD27 / TNFRSF7 protein
Western Blot; Flow Cytometry
Flow Cytometry: Human lymphocytes were stained with FITC-anti human CD27 (CAB11513 (solid line)) or FITC-rabbit IgG isotype control (dashed line). The CAB11513 and CAB11512 monoclonal antibodies cross-block binding, suggesting recongnition of overlapping epitopes.Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human CD27 in WB. Use of this antibody under reducing conditions is not recommended.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD27 and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Aqueous solution containing 0.5% BSA and 0.1% sodium azide
5 μl/Test, 0.2 mg/ml
This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze!Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.