Rabbit monoclonal antibody to human CD27 molecule, FITC-conjugated.
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, and during which, adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling processes. In addition, a proapoptotic protein named SIVA, is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.
Human CD27 / TNFRSF7, Has cross-reactivity in ELISA with Mouse CD27. No cross-reactivity in ELISA with Human TNFRSF8/CD30, Human 4-1BB, Human CD40, Human DR6, Human FAS, Human HVEM/TNFRSF14, Human TNFRI, Human TNFRII, Human BAFF, Human RELT, Mouse HVEM, Mouse FAS, Rat FAS.
Recombinant human CD27 / TNFRSF7 protein
Western Blot; Flow Cytometry
Flow Cytometry: Human lymphocytes were stained with FITC-anti human CD27 (CAB11513 (solid line)) or FITC-rabbit IgG isotype control (dashed line). The CAB11513 and CAB11512 monoclonal antibodies cross-block binding, suggesting recongnition of overlapping epitopes.Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human CD27 in WB. Use of this antibody under reducing conditions is not recommended.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD27 and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Aqueous solution containing 0.5% BSA and 0.1% sodium azide
5 μl/Test, 0.2 mg/ml
This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze!Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow cytometric analysis of anti-human CD27 expression on human whole blood lymphocytesFlow cytometry was performed on a BD FACSCalibur flow cytometry systemPlease refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.