Rabbit monoclonal antibody to human CD27 molecule.
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, and during which, adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling processes. In addition, a proapoptotic protein named SIVA, is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.
Human CD27 / TNFRSF7. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. Mouse CD27, Human TNFRSF8 / CD30, Human 4-1BB, Human CD40, Human DR6, Human FAS, Human HVEM / TNFRSF14, Human TNFRI, Human TNFRII, Human BAFF, Human RELT, Mouse HVEM, Mouse FAS, Rat FAS, Human cell lysate (293 cell line).
Recombinant Human CD27 / TNFRSF7 Protein
Western Blot; ELISA
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human CD27 in WB. Using a DAB detection system, the detection limit for Human CD27 is approximately 1 ng/lane under non-reducing. Use of this antibody under reducing conditions is not recommended.Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect Human CD27. The detection limit for Human CD27 is approximately 0.00975 ng/well.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human CD27 / TNFRSF7.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.