Rabbit monoclonal antibody to mouse Cd34 antigen.
Mouse CD34 molecule is a member of a family of single-pass transmembrane sialomucin proteins, and is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. As a monomeric cell surface antigen, CD34 is highly glycosylated with nine potential N-linked and numerous potential O-linked glycosylation sites in its extracellular domain. Although used clinically in hematopoietic stem cell transplantation for more than 15 years, little is known about the exact function of CD34. It may act as an adhesion molecule in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells, and is also required for T cells to enter lymph nodes via interaction with L-selectin. It was indicated that CD34 is a phosphorylation target for activated PKC, and couples to the hematopoietic adapter protein CrkL, which were involved in CD34 signaling pathways. CD34 is abberantly expressed in many kinds of tumors and is implicated in leukemogenesis.
Recombinant Mouse CD34 Protein
Western Blot; ELISA; Immunohistochemistry
Immunochemistry: IHC-P: 1 - 5 μg/mL.Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Mouse CD34 in WB.Using a DAB detection system, the detection limit for Mouse CD34 is approximately 0.25 ng/lane under reducing conditions.Use of this antibody under non-reducing conditions is not recommended.Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect Mouse CD34. The detection limit for Mouse CD34 is approximately 0.00975 ng/well.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse CD34.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Immunochemical Staining of mouse CD34 in mouse kidney with Rabbit Monoclonal Antibody . Positive staining was localized to vascular endothelial cells.