Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Mouse Anti-Human Carboxypeptidase B1 Monoclonal Antibody

Mouse Anti-Human Carboxypeptidase B1 Monoclonal Antibody

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Cat.No.:
CAB11512MH
Product Overview:
Mouse Monoclonal Antibody to Human Carboxypeptidase B1
Antigen Description:
Human Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Specificity:
Human CPB1 / PCPB. No cross-reactivity in WB and ELISA with mouse CPB1, human CPA1, human CPA2, human CPE and human cell lysate (293 cell line)
Immunogen:
Recombinant human CPB1 protein
Host:
Mouse
Isotype:
Mouse IgG1
Species:
Human
Clone:
3D11D7C13
Applications:
WB; ELISA
Dilution:
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CPB1 in WB. Using a DAB detection system, the detection limit for human CPB1 is approximately 1 ng/lane under non-reducing conditions and reducing conditionsELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human CPB1. The detection limit for human CPB1 is 0.31 ng/well
Preparation:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CPB1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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