Mouse Monoclonal Antibody to Human Clusterin
Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimers disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. CLU / Clusterin is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.
Human Clusterin / Apolipoprotein J / Apo-J. No cross-reactivity in ELISA with Mouse CLU / Clusterin / Apolipoprotein J / Apo-J; Human cell lysate (293 cell line)
Recombinant Human Clusterin / Apolipoprotein J / Apo-J Protein
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CLU in WB. Using a DAB detection system, the detection limit for Human CLU is approximately 20 ng/lane under non-reducing conditions and reducing conditions.ELISA: This antibody can be used at 0.5-1 μg with the appropriate secondary reagents to detect Human CLU. The detection limit for Human CLU is approximately 0.16 ng/well.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human Clusterin / Apolipoprotein J / Apo-J. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.