Rabbit Monoclonal Antibody to Human Dopa Decarboxylase
Dopa Decarboxylase ( DDC ), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinsons disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.
Human DDC / AADC. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Recombinant human DDC protein
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human DDC in WB. Using a DAB detection system, the detection limit for human DDC is approximately 0.125 ng/lane under non-reducing conditions and reducing conditionsELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human DDC. The detection limit for human DDC is approximately 0.00245 ng/well
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human DDC / AADC
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.