Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Mouse Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody

Mouse Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody

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"EGFR" Related Products.
Cat.No.:
CAB11527MH
Product Overview:
Mouse Monoclonal Antibody to Human Epidermal Growth Factor Receptor
Antigen Description:
As a member of the epidermal growth factor receptor tyrosine kinases subfamily, EGF R is a type Ⅰ transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR signaling plays a crucial role in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR homo- or hetero- dimerization, initiates subsequent tyrosine autophosphorylation and various downstream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling has been shown to exert actions on carcinogenesis and disease progression, and thus EGFR was proposed as a target for cancer therapy currently.
Specificity:
Human EGFR / ErbB / HER1. No cross-reactivity in ELISA with Human EGFR/ErbB1; Human ErbB2; Human ErbB3; Human ErbB4; Human cell lysate (293 cell line)
Immunogen:
Recombinant Human EGFR / ErbB / HER1 Protein
Host:
Mouse
Isotype:
Mouse IgG2b
Species:
Human
Clone:
4E3I12D6
Applications:
WB; ELISA
Dilution:
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human EGFR in WB. Using a DAB detection system, the detection limit for Human EGFR is approximately 1 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended.ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human EGFR. The detection limit for Human EGFR is approximately 0.0195 ng/well.
Preparation:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human EGFR / ErbB / HER1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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