Rabbit Monoclonal Antibody to Human Erythropoietin Receptor Protein
Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis , and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR is a type 1 single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPO binding to EPOR triggers auto- or trans-phosphorylation of a protein-tyrosine kinase JAK2 bound to the receptor cytosolic (CT) domain, and subsequently JAK2 activates a variety of intracellular signal substrates including Ras/MAPK, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated EPOR appears to function in erythroid cell survival through preventing the PCD, and is also suggested to play a neuroprotective role for its expression in mammalian brains. Aberrantly expressed EPOR is implicated in pathogenesis of various human diseases including erythroleukemia and familial erythrocytosis.
Human EPOR. Has cross-reactivity in ELISA with Mouse EPOR. No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Recombinant Human EPOR Protein
Western blot; ELISA
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human EPOR in WB. Using a DAB detection system, the detection limit for Human EPOR is approximately 0.5 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions.ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human EPOR. The detection limit for Human EPOR is approximately 0.00245 ng/well.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human EPOR
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.