Rabbit Monoclonal Antibody to Human VEGF-D
Vascular endothelial growth factor D (VEGF-D), also known as C-fos induced growth factor (FIGF), is a vascular endothelial growth factor. This secreted protein is a member of the platelet derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and performs activities in angiogenesis, lymphangiogenesis, and endothelial cell growth. Human VEGF-D is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. It is structurally and functionally similar to VEGF-C, and is secreted as a non-covelent homodimer in a antiparallel fashion. VEGF-D undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. Like VEGFC, it may particularly be involed in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults.
Human VEGF-D / FIGF / VEGFD. No cross-reactivity in ELISA with human VEGF-C / Flt4-Land human cell lysate (293 cell line)
Recombinant human VEGF-D protein
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human VEGF-D in WB. Using a DAB detection system, the detection limit for human VEGF-D is approximately 0.5 ng/lane under non-reducing conditions and 50 ng/lane under reducing conditionsELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human VEGF-D. The detection limit for human VEGF-Dis approximately 0.0049 ng/well
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human VEGF-D.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.