Rabbit Monoclonal Antibody to Human FUT10
Human Alpha--fucosyltransferase 10, also known as Galactoside 3-L-fucosyltransferase 10, Fucosyltransferase 10, Fucosyltransferase X, Fuc-TX, and FUT10, is single-pass type I I membrane protein which belongs to the glycosyltransferase 10 family. Fucosyltransferases are the enzymes transferring fucose from GDP-Fuc to Gal in an alpha1,2-linkage and to GlcNAc in alpha1,3-linkage, alpha1,4-linkage, or alpha1,6-linkage. All fucosyltransferases utilize the same nucleotide sugar, their specificity reside in the recognition of the acceptor and in the type of linkage formed. Fucosyltransferases share some common structural and catalytic features. On the basis of protein sequence similarities, these enzymes can be classified into four distinct families:the alpha-2-fucosyltransferases,the alpha-3-fucosyltransferases,the mammalian alpha-6-fucosyltransferases, andthe bacterial alpha-6-fucosyltransferases. The alpha-3-fucosyltransferases constitute a distinct family as they lack the consensus peptide, but some regions display similarities with the alpha-2 and alpha-6-fucosyltranferases.
Human Fucosyltransferase 10 / FUT10. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Recombinant human FUT10 protein
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human FUT10 in WB. Using a DAB detection system, the detection limit for human FUT10 is approximately 0.5 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditionsELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human FUT10. The detection limit for human FUT10 is approximately 0.078 ng/well
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human FUT10.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.