Rabbit Anti-Rat GPT2 Monoclonal Antibody

• Cat.No.: CAB11548RR
• Product Overview: Rabbit Monoclonal Antibody to Rat GPT2

Specification

• Antigen Description: Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (Gpt), is a pyridoxal enzyme which catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate, and plays a key role in the intermediary metabolism of glucose and amino acids. As a key enzyme for gluconeogenesis, Gpt is a widely-used serum marker for liver injury. Two ALT isoenzymes have been identified, ALT1 and ALT2 (GPT1 and GPT2), which are encoded by separate genes and share significant sequence homology, but differ in their expression patterns. Gpt1 is widely distributed and mainly expressed in intestine, liver, fat tissues, colon, muscle, and heart, in the order of high to low expression level, whereas Gpt2 expression is more restricted, mainly in liver, muscle, brain, and white adipose tissue. It has been reported that hepatic ALT2 protein is approximately four times higher in male rats than in female rats.
• Specificity: Rat GPT2
• Immunogen: Recombinant Rat GPT2 Protein
• Host: Rabbit
• Isotype: Rabbit IgG
• Species: Rat
• Cross Reactivity: 0331
• Clone: A0332
• Applications: WB; ELISA
• Dilution: Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Rat GPt2 in WB.ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat GPt2. The detection limit for Rat GPt2 is approximately 0.039 ng/well.
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Rat GPT2.
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Gpt2 glutamic pyruvate transaminase (alanine aminotransferase) 2 [ Rattus norvegicus ]
• Official Symbol: Gpt2
• Synonyms: GPT2; glutamic pyruvate transaminase (alanine aminotransferase) 2; alanine aminotransferase 2; glutamic pyruvate transaminase 2; glutamic-pyruvate transaminase 2; ALT2; Cc2-5
• Gene ID: 307759
• mRNA Refseq: NM_001012057
• Protein Refseq: NP_001012057
• Pathway: Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: L-alanine:2-oxoglutarate aminotransferase activity