Rabbit Monoclonal Antibody to Human TIMD3 / HAVCR2
Hepatitis A virus cellular receptor 2 ( HAVCR2 ), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. TIM-3 thus serves as the surface molecule that distinguishes between the two effector populations derived from naive CD4(+) T-helper cells. TIM-3 is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. Th1 cells produce cytokines ( IFN-gamma, interleukin-2, TNF-alpha and lymphotoxin ) that are commonly associated with cell-mediated immune responses against intracellular pathogens, delayed-type hypersensitivity reactions, and induction of organ-specific autoimmune diseases. It has been identified that galectin-9 is the Tim-3 ligand, and the TIM-3-galectin-9 pathway may serve to inhibit Th1 cells during a normal immune response and may be crucial for the induction of peripheral tolerance. Dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.
Human TIMD3 / HAVCR2. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA
Recombinant human TIMD3 protein
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human TIMD3 in WBELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human TIMD3. The detection limit for human TIMD3 is approximately 0.0049 ng/well
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human TIMD3.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.