Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Mouse Anti-Human Inter-Cellular Adhesion Molecule 1 Monoclonal Antibody

Mouse Anti-Human Inter-Cellular Adhesion Molecule 1 Monoclonal Antibody

   Share
"ICAM1" Related Products.
Cat.No.:
CAB11556MH
Product Overview:
Mouse Monoclonal Antibody to Human Inter-Cellular Adhesion Molecule 1
Antigen Description:
ICAM1 (Inter-Cellular Adhesion Molecule 1), also known as CD54, is a type of intercellular adhesion molecule constitutely present in low concentrations in the membranes of leukocytes and endothelial cells. As an endothelial and leukocyte-associated transmembrane protein, ICAM1 is well known for its importance in stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration. ICAM1 is a ligand for LFA-1 (integrin). When activated, leukocytes bind to endothelial cells via ICAM1 / LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease.
Specificity:
Human ICAM1 / CD54. No cross-reactivity in WB and ELISA with Human ICAM2 / CD102, Human ICAM3 / CD50,Human VCAM1 / CD106, Human CEACAM1 / CD66a, Human E-selectin / SELE / CD62E / ELAM1, Human cell lysate (293 cell line)
Immunogen:
Recombinant human ICAM-1 protein
Host:
Mouse
Isotype:
Mouse IgG1
Species:
Human
Cross Reactivity:
8H8G9
Clone:
9I9H10
Applications:
WB
Dilution:
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human ICAM1 in WB. Using a DAB detection system, the detection limit for human ICAM1 is approximately 8 ng/lane under non-reducing conditions and 20 ng/lane under reducing conditions
Preparation:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human ICAM1 / CD54 extracellular domain ( rh ICAM1 ; NP_000192.2 ; Met 1- Glu 480 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.