Rabbit Anti-Human IL1B / IL1F2 Monoclonal Antibody
Cat.No. : | CAB11564RH |
Product Overview : | Rabbit Monoclonal Antibody to Human IL1B / IL1F2 |
- Specification
- Gene Information
- Related Products
Antigen Description : | Interleukin 1 (IL-1) is a family of polypeptide cytokines consisting of two agonists, IL-1alpha (IL-1F1) and IL-1beta (IL-1F2) encoded by two distinct genes and perform identical biological functions. IL1α and IL1β binds directly to a high-affinity receptor complex of IL1 RI and IL1 R AcP to initiate the signal transduction. IL1RII also binds to IL1β with high affinity, but on contrast, IL1β activity is inhibited since IL1RII exists as a decoy receptor and negative regulator.IL1R antgonist (IL1RA), also a member of IL-1 family, is identified to interact with IL1R1, preventing IL1α and IL1β to exert actions. IL1β is produced by a variety of cell types, and capable of stimulating thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. Also identified as endogenous pyrogens, IL-1 are involved in the immune and inflammatory responses, and are reported to stimulate the release of prostaglandin and collagenase. IL1β is encoded as a inactive precusor of 269 amino acids with a 116 aa propeptide which is cleaved intracellularly by the cysteine protease, IL1β converting enzyme (Caspase1/ICE) to generate the biologically active cytokine. Lacking of a specific hydrophobic segment suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
Specificity : | Human IL-1 beta / IL1B. No cross-reactivity in WB and ELISA with Human IL1A/ IL1F1, Human IL1R2 / IL1RB, Human cell lysate (293 cell line) |
Immunogen : | Recombinant human ILb protein |
Host : | Rabbit |
Isotype : | Rabbit IgG |
Species : | Human |
Cross Reactivity : | 012 |
Clone : | A013 |
Applications : | WB; ELISA |
Dilution : | Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human IL1bin WB. Using a DAB detection system, the detection limit for human IL1b is approximately 0.5 ng/lane under non-reducing conditions and reducing conditionsDirect ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human IL1b. The detection limit for human IL1b is approximately 0.0049 ng/well |
Preparation : | This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse IL18R1 ( rM IL18R1; NP_032391.1; Met 1 - Gly 326) |
Format : | 0.2 μm filtered solution in PBS with 5% trehalose |
Storage : | This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Gene Name : | IL1B interleukin 1, beta [ Homo sapiens ] |
Official Symbol : | IL1B |
Synonyms : | IL1B; interleukin 1, beta; interleukin-1 beta; IL 1B; IL1 BETA; IL1F2; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; IL-1; IL1-BETA; |
Gene ID : | 3553 |
mRNA Refseq : | NM_000576 |
Protein Refseq : | NP_000567 |
MIM : | 147720 |
UniProt ID : | P01584 |
Chromosome Location : | 2q14 |
Pathway : | African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Apoptosis, organism-specific biosystem; |
Function : | cytokine activity; cytokine activity; growth factor activity; interleukin-1 receptor binding; protein domain specific binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionInflammasome assembly initiated by IL-1β is deciphered using techniques like co-immunoprecipitation, proximity ligation assays, and super-resolution microscopy.
IL-1β's priming of immune cells is dissected through flow cytometry, RNA sequencing, and chromatin immunoprecipitation, revealing its epigenetic and transcriptional effects.
Mass spectrometry-based phosphoproteomics deciphers IL-1β's post-translational modifications, revealing regulatory sites crucial for its activation and signaling.
Bioinformatics tools integrate multi-omics data to provide a comprehensive understanding of IL-1β's roles, revealing its intricate contributions to health and disease.
ChIP-seq and DNA methylation profiling uncover IL-1β's epigenetic impact on target genes, shedding light on its role in shaping chronic inflammatory states.
IL-1β's interactions with downstream effectors are unveiled using dual-luciferase reporter assays, surface plasmon resonance, and co-crystallization studies.
Network analysis integrates omics data to unravel IL-1β's intricate cross-talk with other cytokines, mapping out complex regulatory loops in inflammation.
IL-1β's impact on the tumor microenvironment is studied using intravital microscopy, 3D culture models, and single-cell RNA sequencing to capture dynamic cell behavior.
CRISPR-Cas9 screening identifies IL-1β's non-canonical signaling mediators through functional genomics, revealing alternative pathways in cellular responses.
Single-cell RNA sequencing unveils IL-1β's context-specific responses, exposing cell type-specific signaling and gene expression patterns.
Customer Reviews (3)
Write a reviewEffective in inflammation studies.
High specificity.
Suitable for cytokine assays.
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