Mouse Monoclonal Antibody to Human Layilin
Layilin, a recently characterized transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. As one of the adaptor proteins, talin mediates the interactions between the actin filaments and the cell membrane by binding to integral membrane proteins and to the cytoskeleton. Layilin is a newly identified membrane-binding site for talin in peripheral ruffles of spreading cells, and its adjacent LH2-LH3 tandem arrays in the cytoplasmic domain provide docking sites for talin. Layilin binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. In addition, merlin and radixin have been identified as different intracellular binding partners of Layilin. Accordingly, it has been suggested that Layilin plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction.
HumanLayilin. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Recombinant human Layilin protein
Western blot:This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect layilin in WB. Using a DAB detection system, the detection limit for Layilin is approximately 20ng/lane under reducing conditions. Use of this antibody under non-reducing conditions is not recommended.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human Layilin (rhLayilin; aa 1 - 220; NP_849156.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.Avoid repeated freeze-thaw cycles.