Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Antibody > Monoclonal Antibodies > Mouse Monoclonal Antibody to Human Neuropilin And Tolloid-Like 1

Mouse Monoclonal Antibody to Human Neuropilin And Tolloid-Like 1

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Product Overview:
Mouse Monoclonal Antibody to Human Neuropilin And Tolloid-Like 1
Antigen Description:
Neuropilin and tolloid-like protein 1, also known as brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, NETO1 and BTCL1, is a single-pass type I membrane protein which contains two CUB domains and contains one LDL-receptor class A domain. Isoform 1 and isoform 2 of NETO1 / BTCL1 are retina-specific. Isoform 3 is found in retina as well as at lower levels in adult and fetal brain. NETO1 / BTCL1 interacts with PLZ domains of DLG2, DLG3 and DLG4 via its C-terminal TRV domain. It interacts with GRIN2A and GRIN2B via its CUB domains. NETO1 / BTCL1 is involved in the development and/or maintenance of neuronal circuitry. It is an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. NETO1 / BTCL1 regulates long-term NMDA receptor-dependent synaptic plasticity and cognition in the context of spatial learning and memory.
Human NETO1. No cross-reactivity with human cell lysate ( 293 cell line ) in ELISA.
Recombinant Human NETO1 Protein
Mouse IgG1
Western blot; ELISA
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human NETO1 in WB. Using a DAB detection system, the detection limit for Human NETO1 is approximately 8 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.5-1 μg with the appropriate secondary reagents to detect Human NETO1. The detection limit for Human NETO1 is approximately 0.16 ng/well.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human NETO1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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