Mouse Monoclonal Antibody to Human Neuropilin And Tolloid-Like 1
Neuropilin and tolloid-like protein 1, also known as brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, NETO1 and BTCL1, is a single-pass type I membrane protein which contains two CUB domains and contains one LDL-receptor class A domain. Isoform 1 and isoform 2 of NETO1 / BTCL1 are retina-specific. Isoform 3 is found in retina as well as at lower levels in adult and fetal brain. NETO1 / BTCL1 interacts with PLZ domains of DLG2, DLG3 and DLG4 via its C-terminal TRV domain. It interacts with GRIN2A and GRIN2B via its CUB domains. NETO1 / BTCL1 is involved in the development and/or maintenance of neuronal circuitry. It is an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. NETO1 / BTCL1 regulates long-term NMDA receptor-dependent synaptic plasticity and cognition in the context of spatial learning and memory.
Human NETO1. No cross-reactivity with human cell lysate ( 293 cell line ) in ELISA.
Recombinant Human NETO1 Protein
Western blot; ELISA
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human NETO1 in WB. Using a DAB detection system, the detection limit for Human NETO1 is approximately 8 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.5-1 μg with the appropriate secondary reagents to detect Human NETO1. The detection limit for Human NETO1 is approximately 0.16 ng/well.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human NETO1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.