Rabbit Monoclonal Antibody to Human Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor, Alpha
Cat.No. : | CAB11587RH |
Product Overview : | Rabbit Monoclonal Antibody to Human Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor, Alpha |
- Specification
- Gene Information
- Related Products
Antigen Description : | NF-kappa-B inhibitor alpha, also known as Major histocompatibility complex enhancer-binding protein MAD3, I-kappa-B-alpha, NFKBIA and IKBA, is a cytoplasm and nucleus protein which belongs to the NF-kappa-B inhibitor family. NFKBIA contains five ANK repeats. NFKBIA inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, NFKBIA becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection. |
Specificity : | Human IkB alpha / NFKBIA. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. |
Immunogen : | Recombinant Human IkB alpha / NFKBIA Protein |
Host : | Rabbit |
Isotype : | Rabbit IgG |
Species : | Human |
Clone : | A117 |
Applications : | Western blot; ELISA |
Dilution : | Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human NFKBIA in WB. Using a DAB detection system, the detection limit for Human NFKBIA is approximately 2 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human NFKBIA. The detection limit for Human NFKBIA is approximately 0.00245 ng/well. |
Preparation : | This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human IkB alpha / NFKBIA |
Format : | 0.2 μm filtered solution in PBS with 5% trehalose |
Storage : | This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Gene Name : | NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha [ Homo sapiens ] |
Official Symbol : | NFKBIA |
Synonyms : | NFKBIA; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; NFKBI; NF-kappa-B inhibitor alpha; IkappaBalpha; IKBA; MAD 3; ikB-alpha; I-kappa-B-alpha; nuclear factor of kappa light chain gene enhancer in B-cells; major histocompatibility complex enhancer-binding protein MAD3; MAD-3; |
Gene ID : | 4792 |
mRNA Refseq : | NM_020529 |
Protein Refseq : | NP_065390 |
MIM : | 164008 |
UniProt ID : | P25963 |
Chromosome Location : | 14q13 |
Pathway : | Activated TLR4 signalling, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; |
Function : | NF-kappaB binding; NF-kappaB binding; enzyme binding; heat shock protein binding; identical protein binding; nuclear localization sequence binding; protein binding; transcription factor binding; ubiquitin protein ligase binding; |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThe interaction between NFKBIA protein and NF-κB can be identified by co-immunoprecipitation, bimolecular fluorescence complementation, etc. In addition, these methods can also be used to study the interaction sites and mechanisms of action between them.
Knockdown of the NFKBIA gene leads to increased cell growth arrest and apoptosis, which may be related to the aberrant activation of the NF-κB signaling pathway.
The effect on the expression level and activity of molecules related to the NF-κB signaling pathway can be observed by overexpressing or knocking down NFKBIA protein. In addition, studies can also be performed with specific knockout or mutant cell lines.
Its role in physiological and pathological processes can be studied by specifically knocking out the NFKBIA gene using specific short hairpin RNA or zinc finger nuclease technology.
Studies have shown that the expression level of NFKBIA protein is associated with the occurrence and progression of a variety of diseases. For example, in some cancers, the expression levels of the NFKBIA protein are reduced or missing, leading to aberrant activation of the NF-κB signaling pathway.
When cells are stimulated by inflammation, the NFKBIA protein is phosphorylated, causing its dissociation from NF-κB. After dissociation, NF-κB is transferred to the nucleus and activates the transcription of specific genes. In addition, NFKBIA protein can further regulate the activity of NF-κB by interacting with other proteins.
Customer Reviews (3)
Write a reviewI am very happy with the results, they performed very well in my experiments.
It has high sensitivity and strong specificity.
It is easy to use and requires little preparation before the experiment.
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