Mouse Monoclonal Antibody to Human 5'-Nucleotidase, Ecto
Cat.No. : | CAB11589MH |
Product Overview : | Mouse Monoclonal Antibody to Human 5-Nucleotidase, Ecto |
- Specification
- Gene Information
- Related Products
Antigen Description : | Mouse 5-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium. |
Specificity : | Human CD73 / NT5E. No cross-reactivity with human cell lysate ( 293 cell line ) in ELISA. |
Immunogen : | Recombinant Human CD73 / NT5E Protein |
Host : | Mouse |
Isotype : | Mouse IgG2b |
Species : | Human |
Clone : | 9E11H8 |
Applications : | Western blot; ELISA |
Dilution : | Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect NT5E-His? in WB. Using a DAB detection system, the detection limit for NT5E-His? is approximately 4 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect NT5E-His. The detection limit for NT5E-His is approximately 0.31 ng/well. |
Preparation : | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human CD73 / NT5E. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Format : | 0.2 μm filtered solution in PBS with 5% trehalose |
Storage : | This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Gene Name : | NT5E 5-nucleotidase, ecto (CD73) [ Homo sapiens ] |
Official Symbol : | NT5E |
Synonyms : | NT5E; 5-nucleotidase, ecto (CD73); 5 nucleotidase (CD73) , NT5; 5-nucleotidase; CD73; eN; eNT; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase; NT; NT5; NTE; E5NT; |
Gene ID : | 4907 |
mRNA Refseq : | NM_001204813 |
Protein Refseq : | NP_001191742 |
MIM : | 129190 |
UniProt ID : | P21589 |
Chromosome Location : | 6q14-q21 |
Pathway : | HIF-1-alpha transcription factor network, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Purine catabolism, organism-specific biosystem; |
Function : | 5-nucleotidase activity; ferrous iron binding; hydrolase activity, acting on ester bonds; metal ion binding; nucleotide binding; |
Products Types
◆ Recombinant Protein | ||
NT5E-1160H | Recombinant Human NT5E Protein, His-tagged | +Inquiry |
NT5E-1302H | Recombinant Human NT5E Protein, GST-tagged | +Inquiry |
NT5E-3859H | Recombinant Human NT5E, His tagged | +Inquiry |
NT5E-151H | Recombinant Human NT5E Protein, His-tagged | +Inquiry |
NT5E-435H | Recombinant Human 5'-nucleotidase, ecto (CD73), His-tagged | +Inquiry |
◆ Lysates | ||
NT5E-1082RCL | Recombinant Rat NT5E cell lysate | +Inquiry |
NT5E-2491MCL | Recombinant Mouse NT5E cell lysate | +Inquiry |
NT5E-2073HCL | Recombinant Human NT5E cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (5)
Ask a questionYes, elevated levels of NT5E have been observed in various diseases, making it a potential biomarker for conditions such as cancer and autoimmune disorders.
NT5E-generated adenosine has anti-inflammatory effects, and modulating NT5E activity is being explored as a potential therapeutic strategy for inflammatory conditions.
Yes, NT5E is implicated in autoimmune diseases, and targeting its activity is being investigated for potential therapeutic interventions.
As of my last knowledge update in January 2022, there were no FDA-approved drugs specifically targeting NT5E, but research in this area is ongoing.
NT5E-generated adenosine is neuroprotective, and research is exploring its potential in the treatment of neurodegenerative disorders.
Customer Reviews (3)
Write a reviewthe manufacturer's support plays a crucial role in the successful implementation of trials involving the NT5E protein.
The manufacturer maintains stringent quality control measures to ensure the purity and stability of the protein, minimizing experimental variability and maximizing the accuracy of the data obtained.
Their responsiveness to inquiries and willingness to address concerns contribute to a smooth and productive research experience.
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