Rabbit Monoclonal Antibody to Human Poliovirus Receptor-related 2
CD112, also known as Nectin-2, is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. As a homophilic adhesion molecule, CD112 is widely expressed in human tissues including hematopoietic cells and mediates intercellular junctions of adjacent cells. This protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
Human CD112 / Nectin-2 / PVRL2. No cross-reactivity in ELISA with mouse CD112 / PVRL2 and human cell lysate (293 cell line)
Recombinant human CD112 protein
Western blot; ELISA
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CD112 in WB. Using a DAB detection system, the detection limit for human CD112 is approximately 2 ng/lane under non-reducing conditions and 5 ng/lane under reducing conditionsDirect ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human CD112. The detection limit for human CD112 is approximately 0.00245 ng/well
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD112 extracellular domain
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.