Rabbit Monoclonal Antibody to Human Poliovirus Receptor-related 2

• Cat.No.: CAB11593RH
• Product Overview: Rabbit Monoclonal Antibody to Human Poliovirus Receptor-related 2

Specification

• Antigen Description: CD112, also known as Nectin-2, is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. As a homophilic adhesion molecule, CD112 is widely expressed in human tissues including hematopoietic cells and mediates intercellular junctions of adjacent cells. This protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
• Specificity: Human CD112 / Nectin-2 / PVRL2. No cross-reactivity in ELISA with mouse CD112 / PVRL2 and human cell lysate (293 cell line)
• Immunogen: Recombinant human CD112 protein
• Host: Rabbit
• Isotype: Rabbit IgG
• Species: Human
• Clone: A018
• Applications: Western blot; ELISA
• Dilution: Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CD112 in WB. Using a DAB detection system, the detection limit for human CD112 is approximately 2 ng/lane under non-reducing conditions and 5 ng/lane under reducing conditionsDirect ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human CD112. The detection limit for human CD112 is approximately 0.00245 ng/well
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD112 extracellular domain
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: PVRL2 poliovirus receptor-related 2 (herpesvirus entry mediator B) [ Homo sapiens ]
• Official Symbol: PVRL2
• Synonyms: PVRL2; poliovirus receptor-related 2 (herpesvirus entry mediator B); HVEB; poliovirus receptor-related protein 2; CD112; PRR2; PVRR2; nectin-2; poliovirus receptor-like 2; herpesvirus entry protein B
• Gene ID: 5819
• mRNA Refseq: NM_001042724
• Protein Refseq: NP_001036189
• MIM: 600798
• UniProt ID: Q92692
• Chromosome Location: 19q13.2-q13.4
• Pathway: Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem
• Function: cell adhesion molecule binding; coreceptor activity; protein binding; protein homodimerization activity; protein homodimerization activity; receptor activity