Rabbit Monoclonal Antibody to Mouse SLAM Family Member 7
Mouse SLAM family member 7, also known as CRACC, SLAMF7, CD319, CD2-like receptor-activating cytotoxic cells, and CS1, is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B- cell lines, or T-cell lines. The cytoplasmic domain of SLAMF7 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM)-associated protein (SAP/ SH2D1A). SLAMF7 may play a role in the regulation of B lymphocyte proliferation during immune responses. SLAMF7 is implicated in the activation of NK cell-mediated cytotoxicity. SLAMF7 positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. In the absence of EAT-2, SLAMF7 potently inhibited natural killer cell function. SLAMF7 was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, SLAMF7 can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins.
Mouse SLAMF7 / CRACC. Has cross-reactivity in ELISA with human SLAMF7 / CRACCM; No cross-reactivity in ELISA with human cell lysate (293 cell line)
Recombinant mouse SLAMF7 protein
Western blot; ELISA
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse SLAMF7 in WB. Using a DAB detection system, the detection limit for mouse SLAMF7 is approximately 0.25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommendedDirect ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse SLAMF7. The detection limit for mouse SLAMF7 is approximately 0.00245 ng/well
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant mouse SLAMF7 extracellular domain
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.