Mouse Monoclonal Antibody to Human Sulfotransferase Family, Cytosolic, 2A, Dehydroepiandrosterone-Preferring, Member 1
Hydroxysteroid sulfotransferase ( SULT2A1 ) is a key enzyme in the testicular and hepatic metabolism of 5alpha-androstenone, which is a major component of the off-odor and off-flavor in pork known as boar taint. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. SULT2A1 is a sulfo-conjugating phase II enzyme expressed at very high levels in the liver and intestine, the two major first-pass metabolic tissues, and in the steroidogenic adrenal tissue. SULT2A1 acts preferentially on the hydroxysteroids dehydroepiandrosterone, testosterone / dihydrotestosterone, and pregnenolone and on cholesterol-derived amphipathic sterol bile acids.
Human SULT2A1 / STD. No cross-reactivity with human SULT1B1 and E.coli cell lysate in WB and ELISA
Recombinant human SULT2A1 protein
Western blot; ELISA
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human SULT2A1 in WB. Using a DAB detection system, the detection limit for human SULT2A1 is approximately 1 ng/lane under non-reducing conditions and reducing conditionsDirect ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human SULT2A1. The detection limit for human SULT2A1 is approximately 0.039 ng/well
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human SULT2A1 ( rh SULT2A1 ; Catalog#11411-H07E ; NP_003158.2 ; Ser 2 - Glu 285 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.