Rabbit Monoclonal Antibody to Human Tumor-Associated Calcium Signal Transducer 2
Human TROP2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed by human carcinomas. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type Imembrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. The cytoplasmic tail of TROP2 possesses potential serine and tyrosine phosphorylation sites and a conserved phosphatidyl-inositol binding consensus sequence. TROP2 transduces an intracellular calcium signal and acts as a cell surface receptor, meanwhile is involved in the regulation of cell- cell adhesion. Accordingly, as a widespread stimulator of human cancer growth and a unique marker and causal factor of metastatic cancer, TROP2 is a possible candidate for diagnosis and molecular target therapy. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
Human TROP2 / TACSTD2. No cross-reactivity in ELISA withHuman BCAM / CD239; Human VCAM1 / CD106; Human ICAM1 / CD54; Human CD146 / MCAM / MUC18; Human CD171 / NCAML1 / L1CAM; Human Cadherin-1 / E-cadherin / CDH1 / CD324
Recombinant human TROP2 protein
Immunochemistry; Western blot; ELISA
IHC-P: 1-10 μg/mLWestern blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human TROP2 in WB. Using a DAB detection system, the detection limit for human TROP2 is approximately 0.5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommendedDirect ELISA: This antibody can be used at 0.1- 0.2 μg/mL with the appropriate secondary reagents to detect human TROP2. The detection limit for human TROP2 is 0.00245 ng/well
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human TROP2 extracellular domain
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Immunochemical staining of human TROP2 in human mammary gland with rabbit monoclonal antibody . Positive staining was localized to membrane of alveolus epithelium.