Mouse Monoclonal Antibody to Human TIMP Metallopeptidase Inhibitor 1
Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and the inducible form TIMP1 is a glycoprotein expressed by a variety of cell types. It inhibits most MMPs except membrane-type MMP subfamily by forming non-covalent binary complex, but also interacts with the hemopexin-like (PEX) domain of pro MMP-9. TIMP1 has been identified as a multifunctional molecule with divergent functions. It exhibits keratinocyte and fibroblast growth factor-like activity and acts as a cell survival factor, and additionally participates in tumor metastasis, angiogenesis, inflammatory responses, as well as CNS homeostasis. TIMP1 overexpression may serve to help identify patients with particularly aggressive disease for adjuvant treatments.
Human TIMP1. No cross-reactivity with human cell lysate (293 cell line) in WB and ELISA.
Human cell-derived TIMP1 protein
This antibody can be used at 0.5-1μg/mL with the appropriate secondary reagents to detect TIMP1. The detection limit for TIMP1 is approximately 0.625ng/well.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human TIMP1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.