Rabbit Monoclonal Antibody to Human TIMP Metallopeptidase Inhibitor 1

Cat.No.: CAB11617RH
Product Overview: Rabbit Monoclonal Antibody to Human TIMP Metallopeptidase Inhibitor 1
Antigen Description: Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and the inducible form TIMP1 is a glycoprotein expressed by a variety of cell types. It inhibits most MMPs except membrane-type MMP subfamily by forming non-covalent binary complex, but also interacts with the hemopexin-like (PEX) domain of pro MMP-9. TIMP1 has been identified as a multifunctional molecule with divergent functions. It exhibits keratinocyte and fibroblast growth factor-like activity and acts as a cell survival factor, and additionally participates in tumor metastasis, angiogenesis, inflammatory responses, as well as CNS homeostasis. TIMP1 overexpression may serve to help identify patients with particularly aggressive disease for adjuvant treatments.
Specificity: Human TIMP1. No cross-reactivity with human TIMP2 and human cell lysate ( 293 cell line ) in WB and ELISA
Immunogen: Recombinant human TIMP1 protein
Host: Rabbit
Isotype: Rabbit IgG
Species: Human
Clone: A005
Applications: Western blot; ELISA
Dilution: Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human TIMP1 in WB. Using a DAB detection system, the detection limit for human TIMP1 is approximately 1 ng/lane under non-reducing conditions and 10 ng/lane under reducing conditionsDirect ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human TIMP1. The detection limit for human TIMP1 is approximately 0.00975 ng/well
Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human TIMP1
Format: 0.2 μm filtered solution in PBS with 5% trehalose
Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name: TIMP1 TIMP metallopeptidase inhibitor 1 [ Homo sapiens ]
Official Symbol: TIMP1
Synonyms: TIMP1; TIMP metallopeptidase inhibitor 1; CLGI, TIMP,tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); metalloproteinase inhibitor 1; EPO; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1; EPA; HCI; CLGI; TIMP; FLJ90373;
Gene ID: 7076
mRNA Refseq: NM_003254
Protein Refseq: NP_003245
MIM: 305370
UniProt ID: P01033
Chromosome Location: Xp11.3-p11.23
Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Hemostasis, organism-specific biosystem; IL6-mediated signaling events, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem;
Function: enzyme inhibitor activity; metal ion binding; metalloendopeptidase inhibitor activity; metalloendopeptidase inhibitor activity; protein binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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