"UCHL3" Related Products

Expand
Expand

Rabbit Monoclonal Antibody to Human Ubiquitin Carboxyl-Terminal Esterase L3

Cat.No.: CAB11622RH
Product Overview: Rabbit Monoclonal Antibody to Human Ubiquitin Carboxyl-Terminal Esterase L3
Antigen Description: Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
Specificity: Human UCHL3. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Immunogen: Recombinant Human UCHL3 Protein
Host: Rabbit
Isotype: Rabbit IgG
Species: Human
Clone: A002
Applications: Western blot; ELISA
Dilution: Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human UCHL3 in WB. Using a DAB detection system, the detection limit for Human UCHL3 is approximately 5 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human UCHL3. The detection limit for Human UCHL3 is approximately 0.0195 ng/well.
Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human UCHL3
Format: 0.2 μm filtered solution in PBS with 5% trehalose
Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name: UCHL3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) [ Homo sapiens ]
Official Symbol: UCHL3
Synonyms: UCHL3; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thiolesterase; ubiquitin thioesterase L3; UCH-L3;
Gene ID: 7347
mRNA Refseq: NM_006002
Protein Refseq: NP_005993
MIM: 603090
UniProt ID: P15374
Chromosome Location: 13q21.33
Function: cysteine-type peptidase activity; peptidase activity; ubiquitin binding; ubiquitin thiolesterase activity;

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.