Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Mouse Monoclonal Antibody to Human Ubiquitin Specific Peptidase 7

Cat.No. : CAB11623MH
Product Overview : Mouse Monoclonal Antibody to Human Ubiquitin Specific Peptidase 7
  • Specification
  • Gene Information
  • Related Products
Antigen Description : Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein which belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP0 stimulates lytic infection and the reactivation of quiescent viral genomes. ICP0 interacts very strongly with USP7. USP7-mediated stabilization of ICP0 is dominant over ICP0-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP0-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP0 are expressed.
Specificity : Human USP7 / HAUSP. No cross-reactivity with Baculovirus lysate in ELISA.
Immunogen : Recombinant Human USP7 / HAUSP Protein
Host : Mouse
Isotype : Mouse IgG2b
Species : Human
Clone : 8C10I3
Applications : Western blot; ELISA
Dilution : Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human USP7 (208-560) in WB. Using a DAB detection system, the detection limit for Human USP7 (208-560) is approximately 100 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.5-1 μg with the appropriate secondary reagents to detect Human USP7 (208-560). The detection limit for Human USP7 (208-560) is approximately 0.078 ng/well.
Preparation : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human USP7 / HAUSP. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : USP7 ubiquitin specific peptidase 7 (herpes virus-associated) [ Homo sapiens ]
Official Symbol : USP7
Synonyms : USP7; ubiquitin specific peptidase 7 (herpes virus-associated); HAUSP,ubiquitin specific protease 7 (herpes virus associated); ubiquitin carboxyl-terminal hydrolase 7; ubiquitin thioesterase 7; deubiquitinating enzyme 7; ubiquitin-specific-processing protease 7; herpesvirus-associated ubiquitin-specific protease; Herpes virus-associated ubiquitin-specific protease; ubiquitin specific protease 7 (herpes virus-associated); TEF1; HAUSP;
Gene ID : 7874
mRNA Refseq : NM_003470
Protein Refseq : NP_003461
MIM : 602519
UniProt ID : Q93009
Chromosome Location : 16p13.3
Pathway : FoxO family signaling, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; p53 pathway, organism-specific biosystem;
Function : cysteine-type endopeptidase activity; p53 binding; peptidase activity; protein C-terminus binding; protein binding; protein homodimerization activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity; ubiquitin-specific protease activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
Does USP7 interact with specific protein substrates? 12/02/2022

Yes, USP7 interacts with various protein substrates, including p53, MDM2, and PTEN, influencing their stability and cellular functions.

Can USP7 inhibitors be potential cancer therapeutics? 08/04/2022

Inhibitors targeting USP7 are being explored as potential cancer therapeutics, aiming to disrupt the stability of oncogenic proteins and inhibit cancer cell growth.

Does USP7 have tissue-specific expression patterns? 03/29/2021

USP7 exhibits tissue-specific expression patterns, and its levels may vary in different tissues and cell types.

What is the significance of USP7 in neurodegenerative diseases? 02/03/2020

USP7 has been implicated in neurodegenerative diseases, and its dysregulation may contribute to protein aggregation and neuronal dysfunction.

Are there post-translational modifications of USP7? 06/17/2019

Yes, USP7 undergoes post-translational modifications, including phosphorylation and ubiquitination, which can regulate its activity and stability.

How does USP7 regulate the p53 pathway? 04/24/2018

USP7 regulates the p53 pathway by deubiquitinating and stabilizing p53, preventing its degradation and promoting its transcriptional activity.

Is USP7 involved in the immune response? 02/18/2018

Yes, USP7 plays a role in the immune response by regulating the stability of proteins involved in immune signaling pathways.

Customer Reviews (3)

Write a review
Reviews
10/22/2021

    The product's widespread recognition among researchers made it a preferred choice for many scientific studies.

    06/17/2018

      Positive feedback from other labs and researchers confirmed its standing as a valuable research tool.

      06/28/2016

        The product's respected reputation in the scientific community was a testament to its quality and effectiveness.

        Ask a Question for All USP7 Products

        Required fields are marked with *

        My Review for All USP7 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends