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Mouse Monoclonal Antibody to Human Vitronectin

Cat.No.: CAB11624MH
Product Overview: Mouse Monoclonal Antibody to Human Vitronectin
Antigen Description: Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin consists of three domains: the N-terminal Somatomedin B domain, a central domain with hemopexin homology, and a C-terminal domain also with hemopexin homology. The Somatomedin B domain is a close knit disulfide knot and binds to plasminogen activator inhibitor-1 (PAI-1) and stabilizes it. An RGD sequence which is a binding site for membrane bound integrins is also found in Vitronectin. As a component of platelets, Vitronectin serves to regulate proteolysis initiated by plasminogen activation, interacts with the urokinase receptor, and binds to several serpin serine protease inhibitors as well. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.
Specificity: Human Vitronectin / VTN. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Immunogen: Recombinant human VTN protein
Host: Mouse
Isotype: Mouse IgG2a
Species: Human
Clone: 6H12C11E8
Applications: Western blot; ELISA
Dilution: Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human vitronectin in WB. Using a DAB detection system, the detection limit for human vitronectin is approximately 2 ng/lane under non-reducing conditions and reducing conditionsDirect ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human vitronectin. The detection limit for human vitronectin is approximately 0.039 ng/well
Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human Vitronectin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
Format: 0.2 μm filtered solution in PBS with 5% trehalose
Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name: VTN vitronectin [ Homo sapiens ]
Official Symbol: VTN
Synonyms: VTN; vitronectin; vitronectin (serum spreading factor, somatomedin B, complement S protein); complement S protein; serum spreading factor; somatomedin B; VN; epibolin; S-protein; complement S-protein; serum-spreading factor; V75; VNT;
Gene ID: 7448
mRNA Refseq: NM_000638
Protein Refseq: NP_000629
MIM: 193190
UniProt ID: P04004
Chromosome Location: 17q11.2
Pathway: ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; FOXA1 transcription factor network, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; Inflammatory Response Pathway, organism-specific biosystem;
Function: extracellular matrix binding; heparin binding; integrin binding; polysaccharide binding; scavenger receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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