Mouse Monoclonal Antibody to Human Vitronectin
Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin consists of three domains: the N-terminal Somatomedin B domain, a central domain with hemopexin homology, and a C-terminal domain also with hemopexin homology. The Somatomedin B domain is a close knit disulfide knot and binds to plasminogen activator inhibitor-1 (PAI-1) and stabilizes it. An RGD sequence which is a binding site for membrane bound integrins is also found in Vitronectin. As a component of platelets, Vitronectin serves to regulate proteolysis initiated by plasminogen activation, interacts with the urokinase receptor, and binds to several serpin serine protease inhibitors as well. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.
Human Vitronectin / VTN. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Recombinant human VTN protein
Western blot; ELISA
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human vitronectin in WB. Using a DAB detection system, the detection limit for human vitronectin is approximately 2 ng/lane under non-reducing conditions and reducing conditionsDirect ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human vitronectin. The detection limit for human vitronectin is approximately 0.039 ng/well
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human Vitronectin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.