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Recombinant Human ATP Citrate Lyase, His-tagged
A DNA sequence encoding the human ACLY was expressed, with a polyhistidine tag at the N-terminus. The recombinant human ACLY consists of 1120 amino acids and has a calculated molecular mass of 123 KDa.
ACLY is the primary enzyme responsible for the synthesis of cytosoli cacetyl-CoA in many tissues. The enzyme is composed of two subunits in green plants and is composed of two polymer chains which are polypeptides in human. ATP citrate lyase is the primary cytoplasmic enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues and is also responsible for catalyzing the conversion of citrate and CoA into acetyl-CoA and oxaloacetate, along with the hydrolysis of ATP. A definitive role for ATP citrate lyase in tumorigenesis has emerged from ATP citrate lyase RNAi and chemical inhibitor studies, showing that ATP citrate lyase inhibition limits tumor cell proliferation and survival and induces differentiation in vitro. In vivo, it reduces tumor growth leading to a cytostatic effect and induces differentiation.
Predicted N Terminal
Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0, 10% gly Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
The recombinant human ACLY consists of 1120 amino acids and has a calculated molecular mass of 123 KDa. It migrates as an approximately 110 KDa band in SDS-PAGE under reducing conditions.
1120 amino acids
<1.0 EU per μg protein as determined by the LAL method
>90% as determined by SDS-PAGE
Samples are stable for up to twelve months from date of receipt at -70 oC.
Store it under sterile conditions at -70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen Gene Information
ACLY ATP citrate lyase [ Homo sapiens ]
ACLY; ATP citrate lyase; ATP-citrate synthase; ACL; ATP citrate synthase; ATPCL; CLATP; citrate cleavage enzyme; ATP-citrate (pro-S-)-lyase;
ChREBP activates metabolic gene expression, organism-specific biosystem; Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; Fatty Acyl-CoA Biosynthesis, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem;
ATP binding; ATP citrate synthase activity; binding; ligase activity; metal ion binding; nucleotide binding; succinate-CoA ligase (ADP-forming) activity; transferase activity;
ACLY / acly / ATP citrate lyase protein
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