Active Recombinant Human C2 protein(Met1-Leu752), His-tagged

• Cat.No.: C2-6878H
• Product Overview: Recombinant Human complement component 2 (C2) precursor (NP_000054.2) (Met 1-Leu 752) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Leu752
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5, 5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >100 pmoles/min/μg.
• Molecular Mass: The single-chain form of recombinant human complement component C2 comprises 743 amino acids and has a calculated molecular mass of 82.5 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: C2 complement component 2 [ Homo sapiens ]
• Official Symbol: C2
• Synonyms: C2; complement component 2; complement C2; C3/C5 convertase; complement component C2; CO2; DKFZp779M0311
• Gene ID: 717
• mRNA Refseq: NM_000063
• Protein Refseq: NP_000054
• MIM: 613927
• UniProt ID: P06681