Recombinant Human Kynureninase, His-tagged

• Cat.No.: KYNU-1349H
• Product Overview: A DNA sequence encoding the human KLK3 ( P07288 ) ( Met 1 - Pro 261 ) was fused with a polyhistidine tag at the C-terminus.The recombinant human KYNU consists of 475 amino acids and predicts a molecular mass of 53.7 kDa. The apparent molecular mass of rhKYNU is approximately 47 kDa in SDS-PAGE under reducing conditions.

Specification

• Description: KYNU is a pyridoxal-5-phosphate (PLP)-dependent enzyme that catalyzes the hydrolytic cleavage of l-kynurenine to anthranilic acid and l-alanine. Sequence alignment with other PLP-dependent enzymes indicated that kynureninase is in subgroup IVa of the aminotransferases, along with nifS, CsdB, and serine-pyruvate aminotransferase, which suggests that kynureninase has an aminotransferase fold. KYNU is one of the enzymes involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway.
• Source: Baculovirus-Insect cells
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0, 25% gly. Normally 5 %-8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Molecular Mass: 53.7 kDa
• Protein length: 475 aa
• Endotoxin: 1.0 eu/μg of the protein as determined by the lal
• Purity: >92 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 °C.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: KYNU kynureninase [ Homo sapiens ]
• Official Symbol: KYNU
• Synonyms: KYNU; kynureninase; kynureninase (L kynurenine hydrolase); L kynurenine hydrolase; kynureninase (L-kynurenine hydrolase)
• Gene ID: 8942
• mRNA Refseq: NM_001032998
• Protein Refseq: NP_001028170
• MIM: 605197
• UniProt ID: Q16719
• Chromosome Location: 2q22.2
• Pathway: Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; NAD biosynthesis II (from tryptophan), organism-specific biosystem; Selenium Pathway, organism-specific biosystem; Tryptophan catabolism, organism-specific biosystem; Tryptophan metabolism, organism-specific biosystem
• Function: hydrolase activity; kynureninase activity; kynureninase activity; kynureninase activity; kynureninase activity; protein homodimerization activity; pyridoxal phosphate binding