Active Recombinant Mouse Prl protein(Leu32-Cys228), His-tagged

• Cat.No.: Prl-390M
• Product Overview: Recombinant Mouse PRL (NP_035294.2) (Leu 32-Cys 228) was expressed in Insect Cells,with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: C-His
• Protein length: Leu32-Cys228
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to promote proliferation of INS-1 cells. The ED50 for this effect is typically 50-500 ng/mL.
• Molecular Mass: The recombinant mouse PRL consists of 207 amino acids and has a calculated molecular mass of 23.8 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Prl prolactin [ Mus musculus ]
• Official Symbol: Prl
• Synonyms: PRL; prolactin; Prl1a1; AV290867
• Gene ID: 19109
• mRNA Refseq: NM_001163530
• Protein Refseq: NP_001157002