Chicken Polyclonal antibody to Human PI3K.
Phosphorylates PtdIn. PtdIns4P, and PtdIns(4.5)P2 with apreference for PtdIns(4.5)P2. Phosphoinositide 3-kinases(PI3Ks) phosphorylate the 3-prime OH position of theinositol ring of inositol lipids. They have been implicated asparticipants in signaling pathways regulating cell growth byvirtue of their activation in response to various mitogenicstimuli. PI3Ks are composed of a 110 kDa catalytic subunit,such as PIK3CB and an 85-kDa adaptor subunit.This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants.
Recombinant protein Phosphoinositide-3-kinase, catalytic, beta polypeptide
Human, Mouse, Rat
WB (Recommended starting dilutionfor Western blot analysis is 1:500, for tissue or cell staining1:200, Optimal concentrations and conditions for eachapplication should be determined by the user.)
Preservative: 0.02% Sodium Azide, Constituents: phosphatebuffered saline, pH 7.2.
If slight turbidityoccurs upon prolonged storage, clarify the solution bycentrifugation before use. Working dilution samples shouldbe discarded if not used within 12 hours.
Store at 2–8 °C for up to one week. Forextended storage, store in –20 °C freezer in workingaliquots.Avoid repeated freeze / thaw cycles.
|Function||1-phosphatidylinositol-3-kinase activity; ATP binding; insulin receptor substrate binding; phosphatidylinositol 3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity|