Rabbit Polyclonal antibody to Human Akt.
Akt (proteinkinaseB, PKB) is a serine/threonineprotein kinase. Akt is activated by PI3kinase, which is itself activated by tyrosine kinase(Thr308, Ser473). Akt/PKB controls vital cellular functions such as cell survival/apoptosis, cell cycle progression and glucose metabolism. It also acts down-stream from growth factors and hormones. Akt/PKB can inhibit cell cycle arrest by phosphorylating p21. Also, Akt/PKB may potentiate cell cycle progression through increased translation of cyclinD. Akt/PKB directly affects the apoptosis pathway by, for example, targeting the pro-apoptotic Bcl-2 related protein, BAD. It also can phosphorylate three kinases upstream of SAPK. The SAPK system consists of two groups of kinases, and JNK and p38 MAP kinase pathways. The SAPK pathway is an important target for Akt/PKB regulation to promote cell survival.Akt/PKB is able to regulate cell survival through transcriptional factors(Forkhead, nuclear factor-kB(NF-kB), Murine double minute 2(Mdm2)) that are responsible for pro-as well as anti-apoptotic genes. One of the first physiological targets of PKB to be identified was glycogen synthase kinase-3(GSK3), which has been associated with the control of many cellular processes, including glycogen and protein synthesis, and the modulation of transcription factor activity. The involvement of Akt in disease processes like malignancy, neurodegeneration and abnormal glucose metabolism has also been demonstrated.
A synthetic non-phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-T^P-F-C).
Human, Mouse, Rat
Epitope affinity purified
IHC (dilution: 1:50-1:100); WB (dilution: 1:500-1:1000), Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Preservative: 0.02% Sodium Azide, Constituents: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mMNaCland 50% glycerol.
Store at -20º C, avoid Freeze/Thaw Cycles.
|Function||ATP binding; enzyme binding; identical protein binding; kinase activity; nitric-oxide synthase regulator activity; phosphatidylinositol-3,4,5-trisphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein kinase activity; protein serine/threonine kinase activity|