Recombinant Human Tropomyosin 4, His-tagged

• Cat.No.: TPM4-6899H
• Product Overview: Recombinant human TPM4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 30.7 kDa.

Specification

• Description: TPM4 belongs to the tropomyosin family. Tropomyosins are present in virtually all eukaryotic cells (both muscle and nonmuscle), where they bind actin filaments and function to modulate actin-myosin interaction and stabilize actin filament structure. TPM4 binds to actin filaments in muscle and nonmuscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Molecular Mass: 30.7 kDa (268aa), confirmed by MALDI-TOF
• Protein length: 1-248aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MAGLNSLEAV KRKIQALQQQ ADEAEDRAQG LQRELDGERE RREKAEGDVA ALNRRIQLVE EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRAMK DEEKMEIQEM QLKEAKHIAE EADRKYEEVA RKLVILEGEL ERAEERAEVS ELKCGDLEEE LKNVTNNLKS LEAASEKYSE KEDKYEEEIK LLSDKLKEAE TRAEFAERTV AKLEKTIDDL EEKLAQAKEE NVGLHQTLDQ TLNELNCI
• Purity: >85% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TPM4 tropomyosin 4 [ Homo sapiens ]
• Official Symbol: TPM4
• Synonyms: TPM4; tropomyosin 4; tropomyosin alpha-4 chain; TM30p1; tropomyosin-4
• Gene ID: 7171
• mRNA Refseq: NM_001145160
• Protein Refseq: NP_001138632
• MIM: 600317
• UniProt ID: P67936
• Chromosome Location: 19p13.1
• Pathway: Cardiac muscle contraction, organism-specific biosystem; Cardiac muscle contraction, conserved biosystem; Dilated cardiomyopathy, organism-specific biosystem; Dilated cardiomyopathy, conserved biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem; Hypertrophic cardiomyopathy (HCM), conserved biosystem; Muscle contraction, organism-specific biosystem
• Function: actin binding; calcium ion binding; structural constituent of muscle