Active Recombinant Human Dual Specificity Phosphatase 3, His-tagged
Cat.No. : | DUSP3-2492H |
Product Overview : | Recombinant human DUSP3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 22.6 kDa. |
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Description : | DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl. |
Bio-activity : | Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate. |
Molecular Mass : | 22.6 kDa (205aa), confirmed by MALDI-TOF. |
Protein length : | 1-185aa |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP |
Purity : | >95% by SDS - PAGE |
Applications : | SDS-PAGE |
Storage : | Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/ml (determined by Bradford) |
Publication : |
Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer. (2014)
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Gene Name : | DUSP3 dual specificity phosphatase 3 [ Homo sapiens ] |
Official Symbol : | DUSP3 |
Synonyms : | DUSP3; dual specificity phosphatase 3; vaccinia virus phosphatase VH1 related , VHR; dual specificity protein phosphatase 3; vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; dual specificity protein phosphatase VHR; serine/threonine specific protein phosphatase; VHR; |
Gene ID : | 1845 |
mRNA Refseq : | NM_004090 |
Protein Refseq : | NP_004081 |
MIM : | 600183 |
UniProt ID : | P51452 |
Chromosome Location : | 17q21 |
Pathway : | Activated TLR4 signalling, organism-specific biosystem; ERK/MAPK targets, organism-specific biosystem; ERKs are inactivated, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MAP kinase activation in TLR cascade, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; |
Function : | MAP kinase phosphatase activity; hydrolase activity; phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; |
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DUSP3-1180R | Recombinant Rhesus Macaque DUSP3 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
DUSP3-001HCL | Recombinant Human DUSP3 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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