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Recombinant Human Ubiquitin-Conjugating Enzyme E2D 4 (putative), His-tagged

Cat.No. : UBE2D4-2494H
Product Overview : Recombinant human UBE2D4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 18.8 kDa.
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Description : UBE2D4 belongs to the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl.
Molecular Mass : 18.8kDa (167aa), confirmed by MALDI-TOF.
Protein length : 1-147aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFTTKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM
Purity : >95% by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration : 1mg/ml (determined by Bradford assay)
Gene Name : UBE2D4 ubiquitin-conjugating enzyme E2D 4 (putative) [ Homo sapiens ]
Official Symbol : UBE2D4
Synonyms : UBE2D4; ubiquitin-conjugating enzyme E2D 4 (putative); ubiquitin-conjugating enzyme E2 D4; HBUCE1; ubiquitin-protein ligase D4; ubiquitin carrier protein D4; ubiquitin-conjugating enzyme HBUCE1; FLJ32004;
Gene ID : 51619
mRNA Refseq : NM_015983
Protein Refseq : NP_057067
UniProt ID : Q9Y2X8
Chromosome Location : 7p13
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem;
Function : ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; protein binding; ubiquitin-protein ligase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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